Glossary of DNA Terms

DNA Results Legend

PARENTAGE 30K/100K PV Parent Verified - This animal qualifies by DNA to both the Sire and Dam.
SV Sire Verified - This animal qualifies by DNA to the Sire.
DV Dam Verified - This animal qualifies by DNA to the Dam.
GENETIC CONDITIONS Horned/Polled PP Homozygous Polled
HP Heterozygous
HH Homozygous Horned
Coat Colour EDED Homozygous Black
ee Homozygous Red
EDe Heterozygous Black Hided - Red Carrier
GENETIC DEFECTS Proto Testing PN Proto Normal
PC Proto Carrier
PP Proto Positive
Myostatin Testing
F94L, E226X, NT419
0 Homozygous Free
1 Heterozygous
2 Homozygous Carrier
P Probable for the trait
Leptin Testing CC (lean) Animals have less fat and are therefore less economically favourable
CT Animals are economically intermediate
TT (fat) Animals have more fat and therefore are more economically favourable

30K & 100K Testing

Both the 30K and 100K test deliver parentage results and genomically enhanced EPDs. 

 Low Density SNP Genotyping (GGP uLD; 30,000 SNPs)

  • The GGP uLD assay is an excellent tool for conducting SNP parentage verification and is highly accurate.
  • The main advantage of the GGP uLD assay is that this information can be applied to the prediction equations for a specific breed to generate gEPDs at the lowest cost to the breeder.

High Density SNP Genotyping (GGP 100K; 100,000 SNPs)

  • The Bovine 100K assay is also an excellent tool for conducting SNP parentage verification and is highly accurate.
  • Bovine 100K genotyping is used on highly prolific animals (i.e., AI sires, ET dams, and prolific herd sires) that contribute a great deal of genetic material to the entire herd through their many offspring. The Bovine 100K genotypes are used to develop the prediction equations for a specific breed's gEPD calculation. Furthermore, by including animals from their herd, the prediction equations become that much more accurate for the breeder since their animals are contributing to the development of the calculations.
  • The Bovine 100K genotypes can also be applied to existing prediction equations to generate gEPDs on these animals.

Choose the test that reflects your needs as a producer. If you want to verify which bull is working overtime for you or sell progeny based on pedigree, then the 30K might be all you need. If you want to start incorporating genomics in your breeding plan, then a low-density 30K test such as the GGP uLD is a good choice. If your business is breeding highly prolific animals, the 100K might do you the best service in terms of complete and accurate information. Which test you use depends on your on-farm breeding objective - this should help you meet your herd performance targets, and ultimately be based on profitability for the success and sustainability of your operation.

Myostatin Testing

The F94L myostatin mutation was discovered in ~2007 and is one of the many myostatin mutations. F94L is a partial inactivation of the myostatin gene product, unlike the mutations that circulate in Belgian Blue and Piedmontese that are complete inactivation of the product.

F94L mutation has very high frequency in Limousin cattle and it is fairly unique to Limousin. The F94L mutation is a single nucleotide polymorphism (SNP) that codes for a leucine substitution for phenylalanine in the 94th codon for the myostatin. This single base pair change results in partial inactivation of gene product leading to increased myofibrillar hyperplasia (some believe it's also hypertrophy).

Animals with one or two copies of the mutation have large ribeye area, increased muscularity, improved carcass yields, improved meat tenderness, decreased marbling and subcutaneous fat thickness. Recent work also suggests these animals have lower maintenance requirements due to decreased (relatively) organ mass.

Carriers don't have significant changes in birthweights but typically improved growth and efficiency due to decreased muscle turnover and increased lean tissue growth versus fat accretion.

Additional Resources

Leptin Testing

Leptin is a hormone that regulates feed intake, energy expenditure and whole body energy balance. A SNP in the Leptin gene has been associated with several economically important traits including milk production, weaning weight, backfat, marbling, quality grade, yield grade, dry matter intake, and days on feed. There are 3 Leptin variants:

CC (lean) animals have less fat and therefore, are less economically favourable
CT animals are economically intermediate
TT (fat) animals have more fat and therefore, are more economically favourable